Assessment of nuclear DNA integrity of epididymal spermatozoa following experimental chronic spinal cord injury in the rat.

نویسندگان

  • Ali Reza Talebi
  • Mohammad Ali Khalili
  • Ahmad Hossaini
چکیده

Infertility is considered as one of the major problems associated with spinal cord injury (SCI). However, the exact underlying mechanism is still unknown. Therefore, the main objective of this experimental study was to evaluate the effect of chronic SCI on sperm parameters as well as chromatin integrity and DNA of spermatozoa aspirated from cauda epididymis of rats. Forty-five adult Wistar rats were divided into three groups - SCI, sham, and control. Following laminectomy, SCI was induced onto exposed dura matter (T10). The sham group underwent laminectomy of T10 only, while the control rats were not exposed to any type of injury or medication. The cauda epididymal sperms were aspirated after 8 weeks for analysis of sperm parameters and sperm chromatin integrity with aniline blue (AB), chromomycin A3 (CMA3), sodium dodecyl sulphate (SDS), and acridine orange (AO) tests. The sperm progressive motility and normal morphology of SCI rats were significantly changed when compared with other groups (p < 0.05). In addition, AB as well as CMA3 tests were insignificantly increased in the SCI group when compared with the sham and control groups. However, SDS and AO tests were significantly changed in SCI samples when compared with the sham and control groups (p < 0.001). The results showed that chronic SCI in rat disturbs sperm parameters as well as nuclear maturity and DNA integrity of sperms. Therefore, sperm chromatin structure is compromised in SCI animals as revealed by chromatin structural probes. These alterations may reduce the fertility potential of the male gamete following SCI.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

The Effect of Chronic Spinal Cord Injury on Ultrastructure of Epididymal Sperm in Rat

Purpose: The effects of chronic SCI on ultrastructure of spermatozoa aspirated from epididymis of rats. Materials and Methods: 45 adults Wistar rats were divided into 3 groups of SCI, control and sham. Following laminectomy, SCI was induced with a 15g weight dropped from a distance of 10 cm, onto exposed dura matter at T10 level. Sham group underwent laminectomy of T10 only, while control was n...

متن کامل

P-18: Effects of Ethanol Consumption on Chromatin Condensation and DNA Integrity of Epididymal Spermatozoa In Rat

Background: Alcohol abuse is considered as one of the problems associated with poor semen production and sperm quality. Both acute and chronic alcohol consumption may affect spermatozoal chromatin disorders through apoptosis. Therefore, for the first time, this experimental study was performed to evaluate the effect of ethanol consumption on sperm parameters and chromatin integrity of spermatoz...

متن کامل

تأثیر درازمدت ضایعه نخاعی بر روی پارامترهای اسپرم رت بالغ

Introduction: Spinal cord injury (SCI) is a serious public health problem which seriously affects the victim, family, and even the society. Research studies have shown that 80% of SCI victims are men. In recent years, there have been extensive research works on the effect of SCI (acute and/or chronic) on fertility potential of sperm and spermatogenesis in laboratory animals. SCI may disturb the...

متن کامل

P-2: Evaluation of Epididymal Sperm Quality, DNA Damage and Sperm Maturation Abnormality in Streptozotocin- induced Diabetic Mice

Background: Diabetes mellitus, a condition of chronic hyperglycemia, represents one of the greatest concerns to modern global health. Diabetes is a serious metabolic disorders with numerous complications. It is well known that, elevation of blood glucose levels leads to structural and functional changes in various target tissues and organs. The objective of the present study was to verify if th...

متن کامل

Evaluation of epididymal necrospermia following experimental chronic spinal cord injury in rat

Background: Spinal cord injury (SCI) occurs most often to young men at the peak of their reproductive health. Only 10% of SCI men can father children without medical assistance due to potential impairments in ejaculation and sperm quality. Objective: The main objective of this experimental study was to evaluate the epididymal necrospermiasperm death, after chronic SCI in rat. Materials and Meth...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • International journal of andrology

دوره 30 3  شماره 

صفحات  -

تاریخ انتشار 2007